Comparison Between Salivary and Serum Lactate Dehydrogenase Levels in Patients with Oral Leukoplakia and Oral Squamous Cell Carcinoma - A Pilot Study

Priya Shirish Joshi, Madhuri Chougule, Mahesh Dudanakar, Someshwar Golgire

Abstract


Background: The enzyme Lactate Dehydrogenase (LDH) is found in the cells of almost all body tissues. LDH is believed to vary according to the metabolic requirement of each tissue and alternation in LDH levels have been observed during development, under changing biological conditions, and in response to pathological processes. LDH activity in serum increases as a marker of cellular necrosis. Serum LDH levels have been used as a biochemical marker in diagnosis in various body cancers. The profile of salivary LDH is similar to that found in oral epithelium, indicating that the major source of salivary LDH is probably the oral epithelium-shedding cells. Aims and Objectives: To measure and compare the LDH levels in serum and saliva in patients of oral leukoplakia and oral squamous cell carcinoma. Materials and Methods: Clinically diagnosed seven cases each of Oral Leukoplakia (OL) and Oral Squamous Cell Carcinoma (OSCC) were selected and compared with control. After obtaining the consent of the patient, serum and unstimulated whole saliva was collected and processed for LDH measurement by using total LDH Span Kit. Incisional biopsy of selected cases was performed and clinical diagnosis of the cases was confirmed by histopathological examination. Results: LDH activity increased in serum as well as saliva in patients with oral leukoplakia and oral squamous cell carcinoma in comparison to normal control. Conclusion: Salivary LDH estimation can prove to be a valuable substitute to serum LDH as a biochemical marker, as it is a simple, non-invasive procedure and easily accepted by the patient.


Keywords


Lactate Dehydrogenase; Leukoplakia; Saliva;Serum;Squamous Cell Carcinoma.

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