Cytomorphometric Analysis of Buccal Smears in Treated Leprosy Patients and Healthy Individuals: A Comparative Study

Pooja V K, Anila K, Vanishree M, Santosh Hunasgi, Surekha R, Vardendra Manvikar

Abstract


Leprosy is a chronic multisystem disease caused by Mycobacterium Leprae. Oral manifestations occur in 20-60% of the cases. There is no available literature on oral cytological changes related to leprosy. The aim of the present study is to evaluate the cytological changes in the buccal smears of treated leprosy patients and healthy individuals. Materials and Methods: The study was carried out in 30 treated leprosy patients and 30 healthy individuals. The buccal smears obtained were stained by rapid papanicolaou stain. The cellular and nuclear areas were measured using image analyzer and compared with 30 healthy individuals. Student‘t’ test was used for statistical analysis. Results: Cytomorphometric analysis showed a statistically significant increase in average nuclear area (p<0.0001) and significant decrease in average cellular area (p=0.0014) when compared to healthy individuals. Conclusion: Our study focused on oral cytological changes seen in treated leprosy patients. The changes observed can be attributed to Leprae bacilli or multi drug therapy used for longer duration. Furthermore light has to be thrown with larger sample size for the better understanding of the pathogenesis of these oral cytological changes seen in treated leprosy patients.


Keywords


Leprosy; Oral; Cytomorphometry; Xerostomia; Multidrug Therapy

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References


Kustner EC, Cruz MP, Danis CP, et al. Lepromatous Leprosy: A review and case report. Med Oral Patol Oral Cir Bucal. 2006;VOL:474-9.

Ho CK. Leprosy - A Review. Hong Kong Dermatology & Venereology Bulletin. 1999;7(2):65-70.

Author’s Names to be mentioned. World health organization. Global leprosy situation; Weekly Epidemiological Record. 2009;84:333-40.

Rawalani SM, Gummadapu S, Motwani M, Bhowate R, Rawalani S. Orofacial Lesions in Treated Central Indian Leprosy Patients: A Cross Sectional Study with Review of Literature. Indian J Lepr. 2008;80:161-5.

Costa A, Nery J, Oliveira M, Cuzzi T, Silva M. Oral lesions in leprosy. Indian J Dermatol Venereol Leprol. 2003;69(6):381-5.

Suvarna M, Anuradha C, Kumar KK, Sekhar PC, Lalith Prakash Chandra K, Ramana Reddy BV. Cytomorphometric analysis of exfoliative buccal cells in type II diabetic patients. J Dr NTR Univ Health Sci 2012;1:33-7.

Gururaj N, Sivapathasundaram. Cytological finding in iron deficiency anemia. Indian J Dent Res. 2004;15:126-8.

Boggild AK, Keystone JS, Kain KC. Leprosy: a primer for Canadian physicians. Canadian Med Assoc J. 2004;170(1):71-8.

Bucci F Jr, Mesa M, Schwartz RA, Mc Neil G, Lambert C. Oral lesions In lepromatous leprosy. J Oral Med 1987;42:4-6.

Santos GG, Marcucci G, Marchese LM. Aspectos estomatológicos das lesões específicas e não-específicas em pacientes portadores da moléstia de Hansen. Pesqui Odontol Br. 2000;14(3):268-72.

Scheepers A, Lemmer J, Lownie JF. Oral manifestations of leprosy. Lepr Rev. 2003;64:37-43.

Rendall JR, McDougall AC, Willis LA. Intra-oral temperatures in man with special reference to involvement of the central incisors and premaxillary alveolar process in lepromatous leprosy. Int J Lepr Other Mycobact Dis. 1976;44:462-8.

Scheepers A. Correlation of oral surface temperature and lesions of leprosy. Int J Lepr Other Mycobact Dis.1998;66:214-7.

Marilda Aparecida Milanez Morgado de AbreuI; Nilceo Schwery MichalanyII; Luc Louis Maurice WeckxIII; Dalva Regina Neto PimentelIV; Cleonice Hitomi Watashi Hirata; Maurício Mota de Avelar Alchorne. The oral mucosa in leprosy: A clinical and histopathological study. Rev bras Otorhinolaringol. 2006;72(3):312-6.

Deps P, Lockwood DNJ. Leprosy presenting as immune reconstitution inflammatory syndrome: proposed definitions and Classification. Lepr Rev. 2010;81:59–68.


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