Cytomorphometric Analysis of Buccal Smears in Treated Leprosy Patients and Healthy Individuals: A Comparative Study

Pooja V K, Anila K, Vanishree M, Santosh Hunasgi, Surekha R, Vardendra Manvikar


Leprosy is a chronic multisystem disease caused by Mycobacterium Leprae. Oral manifestations occur in 20-60% of the cases. There is no available literature on oral cytological changes related to leprosy. The aim of the present study is to evaluate the cytological changes in the buccal smears of treated leprosy patients and healthy individuals. Materials and Methods: The study was carried out in 30 treated leprosy patients and 30 healthy individuals. The buccal smears obtained were stained by rapid papanicolaou stain. The cellular and nuclear areas were measured using image analyzer and compared with 30 healthy individuals. Student‘t’ test was used for statistical analysis. Results: Cytomorphometric analysis showed a statistically significant increase in average nuclear area (p<0.0001) and significant decrease in average cellular area (p=0.0014) when compared to healthy individuals. Conclusion: Our study focused on oral cytological changes seen in treated leprosy patients. The changes observed can be attributed to Leprae bacilli or multi drug therapy used for longer duration. Furthermore light has to be thrown with larger sample size for the better understanding of the pathogenesis of these oral cytological changes seen in treated leprosy patients.


Leprosy; Oral; Cytomorphometry; Xerostomia; Multidrug Therapy

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