Effect of Green Tea on Salivary Ph and Streptococcus Mutans Count in Healthy Individuals

Sangameshwar Manikya, Vanishree M, Surekha R, Santosh Hunasgi, Anila K, Vardendra Manvikar


Aim: Green tea is a well known, healthy beverage and is an important source of polyphenol antioxidants. Beneficial effects of green tea include such as protection against dental caries, periodontal disease and tooth loss and found that can a decrease in streptococcus mutans count as well as increase in pH. Hence the present study was carried out to evaluate and compare pH of saliva before and after the intake of green tea and to evaluate the role of green tea on growth of oral bacteria in culture using saliva. Material and Methods: Unstimulated saliva was collected from 30 healthy individuals aged between 20-30 years. The pH of saliva was determined before, immediately after and 10 min after drinking green tea using pH electrode. The microbial load was assessed using nutrient agar and mitis salivarius agar by collecting saliva before and immediately after intake of green tea. Data were analyzed using ANOVA followed by post hoc dunnet multiple comparison test. p<0.05 is considered as statistically significant. Results: There was significant difference between salivary pH before, immediately after and after 10 min (p<0.0001) of intake of green tea. There was significant difference between salivary streptococcus mutans count before and after (p<0.001) intake of green tea. Conclusion: The result of the present study has proved that consumption of green tea inhibit salivary Streptococcus mutans count and cause reduction of pH in saliva. So, it could be advisable to encourage the regular consumption of this widely available, tasty and inexpensive beverage as an interesting alternative to other drinks.



Green Tea; Salivary Ph; Streptococcus Mutans; Saliva; Oral; Dental Caries

Full Text:



Cabrera C, Artacho R, Gimenez R. Beneficial effects of green tea – a review. J Am Coll Nutr. 2006;25(2):79–99.

Narotzki B, Reznick AZ, Aizenbud D, Levy Y. Green tea: A promising natural product in oral health. Arch Oral Biol. 2012;57:429-35.

Cooper R, More D, Morre M. Medicinal benefits of green tea. J Altern Complement Med. 2005;11(3):521-8.

Hamtion-miller JMT. Anti-cariogenic properties of tea (Camellia sinesis). J Med Microbiol. 2001;50:299-302.

Tehrani MH, Asghari G, Hajiahmadi M. Comparing Streptococcus mutans and Lactobacillus colony count changes following green tea mouth rinse or sodium fluoride mouth rinse use in children (Randomized double-blind controlled clinical trial). Dent Res J. 2011;8:S58-63.

Xin Xu, Xue D, Christine D. Tea catechin epigallocatechin gallate inhibits streptococcus mutans biofilm formation by suppressing gtf genes. Arch Oral Biol. 2012;57:678-83.

Shafer, Hine, Levy. Textbook of Oral Pathology. 6th Ed. Elsevier; 2009. 431-433 p.

Abd Allah AA, Ibrahium MI, Abd Allah SM, Amin MA. Antimicrobial effect of tea and tea with milk beverages on oral streptococcus mutans and lactobacilli. World Appl Sci J. 2012;19(9):1327-34.

Awadalla HI, Rgab MH, Bassuoni MW, Fayed MT, Abbas MO. Evaluation of the effect of green tea on dental caries and composite restorations. TAF Prev Med Bull. 2011;10(3):269-74.

Taylor PW, Hamtion-miller JMT, Stapleton PD. Antimicrobial properties of green tea catechins. Food Sci Technol Bull. 2005;2:71-81.

Eric WC, Eu YS, Yon PL. Antioxidant and antibacterial properties of green, black and herbal teas of Camellia sinensis. Pharmacognosy Res. 2011;3:266-72.

Takashi O. Anti-caries effects of tea polyphenols. Food Ingredients J. 2005;210:325-30.

Awadalla HI, Ragab MH, Bassuoni MW, Fayed MT, Abbas MO. A pilot study of the role of green tea use on oral health. Int J Dent Hyg. 2011;9(2):110-6.

Chatterjee A, Saluja M, Agarwal G, Alam M. Green tea: A boon for periodontal and general health. J Indian Soc Periodontal 2012;16:161-7.

Deshpande N, Deshpande A, Mafoud S. Evaluation of intake of green tea on gingival and periodontal status: An experimental study. J Interdiscip Dentistry 2012;2:108-12.


  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.

Indexed In Ebscohost Index Copernicus UlrichsWebOpenJGateGoogle Scholar DOAJ Gale Cengage Learning Research Gate
System Developed By Stanford University Arizona State University Simon Fraser University Canadian Centre For Studies In Publishing University of British Columbia - Faculty of Education
W3C XHTML v1.0 Verified W3C verified Valid CSS v2.1 PHP5 Enabled Made On Mac Apache Enabled MySQL Powered
Published by Celesta Software Pvt Ltd