Modified Gallego’s Stain as Differential Stain for Oral Hard Tissues in Oral Pathology: A Preliminary Report
Objectives: Differential staining of hard tissues seen in tooth, bone and pathological lesions to gain insight into their nature and also to confirm histopathological diagnosis obtained via routine staining procedures. Materials and Methods: Initially, normal (control) tissues were stained to study the staining properties of hard tissue, using H&E (except ground section) which was followed by modified gallego’s staining of soft tissue biopsy, decalcified sections as well as ground sections. Pathological lesions containing hard tissues were also stained similarly to confirm the nature of hard tissues. Results: In a properly stained slide cementum stains red and dentine and bone stains green and enamel stains pink in color. The shade of color differs with the amount of mineralization of the hard tissues. Conclusion: Histopathological diagnosis is confirmed by Gallego’s staining, also the colors of hard tissue components in the control corresponded to those of the pathological lesions. Clinical Relevance: The stain can be successfully used to differentiate various types of oral hard tissues in oral pathology using decalcified section, soft tissue biopsies and as well as ground sections which is rarely used for histochemical stains.
Lillie, R. D.: Histopathologic Technique, Philadelphia and Toronto, 1948, p. 203.German, WV. M.: Hortega's Silver Impregnation Methods Am. J. Clia. Path. (Tech. Supply) 3:13, 1939.
Charles A Waldron and Joseph S.Giansanti. Benign and fibro-osseus lesions of the jaws: a clinical-radiologic-histologic review of 65 cases part I fibrous dysplasia of the jaws. Oral surgery 1973, 35:190-201
C.F.A Culling. Histopathological and Histochemical Techniques. London: Butterworth.p63-72
Robert J. Levey. A Modified Gallego's Iron Fuschin Stain as a Differential Stain for Cementum University of Pennsylvania College of Dentistry, Philadelphia, Pa
GS Kumar(2010). Orban’s Oral Histology and Embroyology. Mosby, New Delhi.
Putns S and Desa D.J Aplication of modified Atwood’s stain to study the decalcified section of the bone sections. Technical methods 1977: 900-903.
Roderick A Cawson ,John W Evenson editors. Oral pathology and Diagnosis; New York Saunders.
Katherine A Rider and Lisa M Fleck. Diffrentiation of bone and soft tissue in formalin fixed, paraffin embedded tissue by using methylene-blue/acid-fuschin. J of Analytical and Quantitative Cytology & Histology 2004; 26:246-48
Sonone,A. Hande, M. Chaudhary, R. Bonde,A. Sheorain&N. Agni. Adenoid ameloblastoma with dentinoid and ghost cells. A composite odontogenic tumour: a rare case report and review of the literature. Oral Surgery.2010.
R.Rajendran. Cysts and Tumors Of Odontogenic Origin.In: Shafer,Hines,Levey: Textbook of oral pathology; Elsevier 2009,6th edition p282-83.
Jeffery C.B Stewart.Benign Non Odontogenic tumors. In:Regezi,Sciuba,Jordan. Clinical pathologic Correlation. Saunders 2003,4th Edition p 289-92.
Neville, Damm, Allen, Bouquot. Oral and Maxillofacial pathology. India: Saunders. p410-13.
Charles A Waldron and Joseph S.Giansanti. Benign and fibro-osseus lesions of the jaws: a clinical-radiologic-histologic review of 65 cases part I fibrous dysplasia of the jaws. Oral surgery 1973, 35:340-45.
Anne Burkhardt. Dentine formation is the so- called “fibro-osteo-cemental” lesions of the jaws: histologic, electron, microscopic and immunohistochemical investigations. Oral Surgery Oral Medicine Oral Pathology. May 2011 22.214.171.124:8080/jspui/bitstream/123456789/1337/1/9.pdf
Harsh mohan. Essential Pathology for dental students. 2nd Edition. Jaypee Publication 2002.
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution 3.0 License.
|Published by Celesta Software Pvt Ltd|